Sprecher
Beschreibung
The spread of multidrug-resistant bacteria is largely driven by the overuse and misuse of antibiotics,1 with the aggravating factor of biofilm formation.2 Because the extracellular biofilm matrix acts as a protective barrier, treating these infections is highly challenging, highlighting the need for alternative therapeutic strategies. Lipid-based delivery systems are among the most widely used strategies to overcome multidrug-resistant and biofilm-related infections because of their safety, biodegradability, and favorable physicochemical properties.3 in this context, bacterial extracellular vesicles may represent a promising alternative therapeutic platform. Here, we have characterized the extracellular membrane vesicles produced by the Shewanella vesiculosa bacterium as a possible platform for delivering antibiotics and obtaining information about the vesicles' dimensions and molecular weight. In addition, we have reconstructed the outer membrane of this Gram(-) bacteria, considering the different components, that is, the lipid part, the lipopolysaccharides (LPS), and the capsular polysaccharides (CPS). The information obtained from the chemical-physical characterization of the membrane that mimics the bacterium has allowed us to optimize the preparation of an innovative delivery system, capable of acting as a Trojan horse.
References
1. A. PArmanik, et al., Curr. Microbiol. 2022
2. E. M. Darby. et al., Nat. Rev. Microbiol. 2023
3. X. Lv, et al., Small 2022