Beschreibung
Glycolipids are known to stabilize biomembrane multilayers through preferential sugar-sugar interactions that act as weak transient membrane cross-links. Here, in order to obtain structural insights into this phenomenon, we utilize neutron reflectometry in combination with a floating lipid bilayer architecture that brings two glycolipid-loaded lipid bilayers to close proximity. We find that selected glycolipids with di-, or oligosaccharide headgroups affect the inter-bilayer water layer thickness and appear to contribute to the stability of the double-bilayer architecture by promoting adhesion of adjacent bilayers even against induced electrostatic repulsion. However, we do not observe any redistribution of glycolipids that would maximize the density of sugar–sugar contacts. In addition, we use small-angle and quasi-elastic neutron scattering on oligolamellar phospholipid vesicles containing defined glycolipid fractions in order to elucidate the influence of glycolipids on membrane mechanics and dynamics. Small-angle neutron scattering (SANS) reveals that the oligolamellar vesicles (OLVs) obtained by extrusion are polydisperse with regard to the number of lamellae, n, which renders the interpretation of the quasi-elastic neutron spin echo (NSE) data nontrivial. To overcome this problem, we introduce a method to model the NSE data in a rigorous fashion based on the obtained histograms of n and on their q-dependent intensity-weighted contribution. This procedure yields meaningful values for the bending rigidity of individual lipid membranes and insights into the mechanical coupling between adjacent membrane lamellae, including the effect of the glycolipids.
