Sprecher
Beschreibung
Polysaccharide–protein complexes represent attractive platforms for the encapsulation and delivery of hydrophobic bioactive compounds owing to their biocompatibility, biodegradability, and tunable physicochemical properties. In this study, complexes were formed via electrostatic co-assembly of bovine serum albumin (BSA) with oppositely charged κ- and λ-carrageenan. The effect of carrageenan type and vitamin D3 (VD3) loading on the structural properties and stability of the assemblies was investigated under different conditions.
Structural characterization using scattering and spectroscopic techniques showed complex formation in acidic conditions with similar swollen hierarchical organization, in wich protein constituted the dominant structural component. Incorporation of Vitamin D3 induced structural reorganization of the initially formed complexes, leading to the formation of locally denser domains and larger associates with enhanced stability at neutral pH.
These findings provide insight into the structure and stability of protein–polysaccharide assemblies and their potential as carriers for hydrophobic bioactive compounds.